Quick Tips and Tricks for RNA Isolation Using RNA Extraction Kit
RNA extraction is not an easy task, but you have to deal with various challenges, including RNA degradation, purity levels, low yield, or contaminated DNA. However, based on the types of samples, each of them has unique features that demand special attention. For example, microbes have tough cells that require chemical analysis, while plants have inhibitors that may co-precipitate with RNA and inhibit downstream analysis.
The focus of this article is to discuss the best RNA isolation tips or tricks using RNA isolations kits or RNA extraction kit to maximize the recovery of high-quality, DNA-free RNA.
RNA must be stabilized after collection!
RNA is not always stable, that may lead to quality issues later. A few samples may contain RNases which rapidly and efficiently degrade RNA. To avoid these problems, it would be great to stabilize the RNA first at the time of collection.
For this purpose, you may use different techniques, but all of the researchers may not have quick access to stabilization techniques at the moment of collection. The best idea is to use RNA isolation kits here that can be used to stabilize the sample at the time of its collection. And it is easy to use and apply when compared to other available techniques.
Complete Sample Lysis is a must-have requirement!
At the time of RNA extraction, the best idea to maximize RNA yield and quality is complete sample lysis. However, all samples are not susceptible to the lysis regimen. For example, if we talk about the blood cells or microbial cells, they cannot be lysed efficiently. A traditional lysis technique may not always be effective. Here, you can use RNA extraction kits to speed up the process with maximum quality techniques.
Choosing RNA Extraction Kits
Knowing that RNA isolation and RNA extraction is not a single-step process, multiple RNA isolation kits and RNA extraction kits are available in the market to help you with the process. You have to go with the best one to ease the process that can assure maximum quality results. You will be amazed to know RNA kits are also available in different formats, and you have to select the option as per your requirements.
You don’t have to panic even if you don’t know anything about RNA kits as the Companies always give technical support to help you with the process. The team makes sure that you could have the best experience during RNA extractions in any workflow while using RNA kits for sample Lysis or avoid any DNA contamination.
Mastering RNA Extractions!!
So, high-quality RNA can be extracted from any sample by mastering three simple techniques, stabilize the RNA at the moment of collection, perform complete sample lysis, and choose the RNA isolation kit and RNA extraction kit wisely. By following these proven tips and tricks, you could become an RNA extraction master in a matter of minutes!
